MP33-18 LOSS OF FOXA2 IN NEUROENDOCRINE PROSTATE CANCER LEADS TO INDUCTION OF FUNCTIONAL ANDROGEN RECEPTOR

You have accessJournal of UrologyProstate Cancer: Basic Research & Pathophysiology I (MP33)1 Sep 2021MP33-18 LOSS OF FOXA2 IN NEUROENDOCRINE PROSTATE CANCER LEADS TO INDUCTION FUNCTIONAL ANDROGEN RECEPTOR Zachary M. Connelly, Shu Yang, Siyuan Cheng, and Xiuping Yu ConnellyZachary Connelly More articles by this author , YangShu Yang ChengSiyuan Cheng YuXiuping View All Author Informationhttps://doi.org/10.1097/JU.0000000000002042.18AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Prostate cancer (PCa) is the leading diagnosed in American men. Current treatments for PCa include surgery, radiation, hormone therapy. The gold standard therapeutic interventions treatment with anti-androgens; however, can relapse castration-resistant eventually neuroendocrine prostate (NEPCa). NEPCa most aggressive form a life expectancy less than year following diagnosis no available treatment. Hallmarks loss androgen receptor (AR), expression neuronal markers such as synaptophysin, chromogranin A, FOXA2, well morphological changes resembling stem-like phenotype. pioneer transcription factor expressed embryonically silenced until phenotype occurs. Understanding roles crucial understanding development progression. METHODS: was stably knocked down PC3 cells (PC3/FOXA2-KD). Additionally, overexpressed adenocarcinoma (LNCaP). were subjected RNA protein analysis. Immunofluorescence used visualize AR. Further downstream AR target genes mRNA RESULTS: When cells, increased at both transcript levels. LNCaP where overexpressed, levels decreased. In AR-null down, localized nucleus upon stimulation (Figure 1). Furthermore, stimulation, PC3/FOXA2-KD stimulated including PSA TMPRSS2. Overall, when re-expressed, shows proper nuclear localization, lastly functional. CONCLUSIONS: mechanisms how progresses into loses major remains unclear. We provide some first evidence inversely correlates expression. By further FOXA2’s role through its regulation expression, previous FOXA2-positive/AR-null may be susceptible anti-androgens again. Source Funding: FWCC/Foundation Legacy Funds XY Funding, Office SEED awards, Department Biochemistry Molecular Biology © 2021 Urological Association Education Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e613-e614 Advertisement Copyright Permissions© Inc.MetricsAuthor Information Expand Loading ...